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Zielona kawa na odchudzanie

TitleZielona kawa na odchudzanie
Publication TypeJournal Article
Year of Publication1975
AuthorsSerov, O. L., Manchenko G. P., & Khlebodarova T. M.
JournalBiokhimii͡a (Moscow, Russia)
Start PageOdchudzanie
Date Published1975 Nov-Dec
Keywordsodchudzanie, zielona kawa

Purification procedure of electrophoretic variants FF and SS of 6-phosphogluconate dehydrogenase (6-PGD) is described. The method includes (NH4)2SO4 fractionation and chromatography on DEAE- and CM-celluloses. Isoenzymes were purified about 5000 fold, and were found to be homogenous by disc electrophoresis in 7% polyacrylamide gel. It was found by comparative studies of activities of variants FF and SS, that pH optimum was 8.2 for variant FF and 8.8 for variant SS. Km for 6-phosphogluconate were found to be 17,5-10(-5) M for variants SS and FF respectively.

Short Titleit manager
Alternate JournalBiokhimiia
Refereed DesignationRefereed
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