@article {1236, title = {Zielona kawa na odchudzanie}, journal = {Biokhimii͡a (Moscow, Russia)}, volume = {40}, year = {1975}, month = {1975 Nov-Dec}, pages = {1233-6}, chapter = {Odchudzanie}, abstract = {Purification procedure of electrophoretic variants FF and SS of 6-phosphogluconate dehydrogenase (6-PGD) is described. The method includes (NH4)2SO4 fractionation and chromatography on DEAE- and CM-celluloses. Isoenzymes were purified about 5000 fold, and were found to be homogenous by disc electrophoresis in 7\% polyacrylamide gel. It was found by comparative studies of activities of variants FF and SS, that pH optimum was 8.2 for variant FF and 8.8 for variant SS. Km for 6-phosphogluconate were found to be 17,5-10(-5) M for variants SS and FF respectively.}, keywords = {odchudzanie, zielona kawa}, issn = {0320-9725}, url = {http://www.topsuplementy.com/green-coffee-plus-tabletki-na-odchudzanie-zielona-kawa/}, author = {Serov, O L and Manchenko, G P and Khlebodarova, T M} }