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%0 Journal Article
%J Biokhimii͡a (Moscow, Russia)
%D 1975
%T Zielona kawa na odchudzanie
%A Serov, O L
%A Manchenko, G P
%A Khlebodarova, T M
%K odchudzanie
%K zielona kawa
%N 6
%P 1233-6
%U http://www.topsuplementy.com/green-coffee-plus-tabletki-na-odchudzanie-zielona-kawa/
%V 40
%X Purification procedure of electrophoretic variants FF and SS of 6-phosphogluconate dehydrogenase (6-PGD) is described. The method includes (NH4)2SO4 fractionation and chromatography on DEAE- and CM-celluloses. Isoenzymes were purified about 5000 fold, and were found to be homogenous by disc electrophoresis in 7% polyacrylamide gel. It was found by comparative studies of activities of variants FF and SS, that pH optimum was 8.2 for variant FF and 8.8 for variant SS. Km for 6-phosphogluconate were found to be 17,5-10(-5) M for variants SS and FF respectively.
%8 1975 Nov-Dec

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