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@article {1270,
	title = {A System for Dosage-Based Functional Genomics in Poplar.},
	journal = {The Plant cell},
	volume = {27},
	year = {2015},
	month = {2015 Sep},
	pages = {2370-83},
	abstract = {Altering gene dosage through variation in gene copy number is a powerful approach to addressing questions regarding gene regulation, quantitative trait loci, and heterosis, but one that is not easily applied to sexually transmitted species. Elite poplar (Populus spp) varieties are created through interspecific hybridization, followed by clonal propagation. Altered gene dosage relationships are believed to contribute to hybrid performance. Clonal propagation allows for replication and maintenance of meiotically unstable ploidy or structural variants and provides an alternative approach to investigating gene dosage effects not possible in sexually propagated species. Here, we built a genome-wide structural variation system for dosage-based functional genomics and breeding of poplar. We pollinated Populus deltoides with gamma-irradiated Populus nigra pollen to produce >500 F1 seedlings containing dosage lesions in the form of deletions and insertions of chromosomal segments (indel mutations). Using high-precision dosage analysis, we detected indel mutations in \~{}55\% of the progeny. These indels varied in length, position, and number per individual, cumulatively tiling >99\% of the genome, with an average of 10 indels per gene. Combined with future phenotype and transcriptome data, this population will provide an excellent resource for creating and characterizing dosage-based variation in poplar, including the contribution of dosage to quantitative traits and heterosis.},
	issn = {1532-298X},
	doi = {10.1105/tpc.15.00349},
	author = {Henry, Isabelle M and Zinkgraf, Matthew S and Groover, Andrew T and Comai, Luca}
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